3QID
Crystal structures and functional analysis of murine norovirus RNA-dependent RNA polymerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Detector technology | CCD |
Collection date | 2009-04-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 120.557, 196.262, 109.339 |
Unit cell angles | 90.00, 114.23, 90.00 |
Refinement procedure
Resolution | 49.070 - 2.500 |
R-factor | 0.2016 |
Rwork | 0.199 |
R-free | 0.24490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sh2 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.247 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.096 | 0.096 |
Number of reflections | 78636 | |
<I/σ(I)> | 21.5 | 21.5 |
Completeness [%] | 99.2 | 99.2 |
Redundancy | 7.6 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 1.26M (NH4)2SO4, 100mM cacodylate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |