3QG5
The Mre11:Rad50 complex forms an ATP dependent molecular clamp in DNA double-strand break repair
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9774 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 105.190, 187.000, 300.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.997 - 3.400 |
| R-factor | 0.2602 |
| Rwork | 0.258 |
| R-free | 0.29520 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 0.872 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 3.400 |
| Number of reflections | 40428 |
| Completeness [%] | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 293 | 9 % PEG-6000, 5% MPD, 0,1M HEPES, pH 7.9, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
