3QEH
Crystal structure of human N12-i15, an ADCC and non-neutralizing anti-HIV-1 Env antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979464 |
Spacegroup name | P 43 |
Unit cell lengths | 125.121, 125.121, 149.902 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.590 |
R-factor | 0.21797 |
Rwork | 0.216 |
R-free | 0.25393 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.772 |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.680 |
High resolution limit [Å] | 2.590 | 2.590 |
Rmerge | 0.145 | 0.783 |
Number of reflections | 71539 | |
<I/σ(I)> | 16.3 | 2.7 |
Completeness [%] | 99.0 | 100 |
Redundancy | 7.6 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 01 M HEPES sodium, pH 7.5, 2% v/v PEG 400, 2 M Ammonium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 294K |