3Q8S
Human DNA polymerase iota incorporating dTTP opposite 8-oxo-guanine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 97.494, 97.494, 201.899 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.479 - 2.090 |
R-factor | 0.2281 |
Rwork | 0.227 |
R-free | 0.26590 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.192 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.130 |
High resolution limit [Å] | 2.090 | 2.090 |
Number of reflections | 32497 | |
Completeness [%] | 94.5 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291.15 | 15% PEG 5000MME, 0.15M NH4SO4, 0.1M MES 6.5, 2.5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |