3Q6U

Structure of the apo MET receptor kinase in the dually-phosphorylated, activated state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-02
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	39.720, 63.808, 112.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.460 - 1.600
R-factor	0.1851
Rwork	0.184
R-free	0.21440
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	BUSTER-TNT (BUSTER-TNT 2.5.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.500
High resolution limit [Å]	1.450	3.120	1.450
Rmerge	0.058	0.034	0.778
Number of reflections	50426
<I/σ(I)>	13
Completeness [%]	97.6	99.2	88.8
Redundancy	6.6	6.6	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	15.6 mg/ml protein mixed in 1:1 ratio with reservoir containing 150 mM malic acid, 20% PEG3350., pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K