3Q5E

crystal structure of human Atlastin-1 (residues 1-447) bound to GDP, crystal form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-04
Detector	ADSC QUANTUM 210
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	104.394, 133.549, 175.835
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.544 - 3.013
R-factor	0.2003
Rwork	0.197
R-free	0.27310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.248
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Number of reflections	48872
Completeness [%]	99.7	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	20% PEG3350 and 0.15 M ammonium phosphate dibasic, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K