3Q3Z
Structure of a c-di-GMP-II riboswitch from C. acetobutylicum bound to c-di-GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-26 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.644, 70.285, 86.037 |
| Unit cell angles | 90.00, 98.86, 90.00 |
Refinement procedure
| Resolution | 38.070 - 2.510 |
| R-factor | 0.1811 |
| Rwork | 0.178 |
| R-free | 0.23620 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.170 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 | 0.841 |
| Number of reflections | 15422 | |
| <I/σ(I)> | 18.4 | 2 |
| Completeness [%] | 98.1 | 100 |
| Redundancy | 5.7 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 13% PEG8000, 200 mM magnesium acetate, 100 mM sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
