3Q36
Crystal structure of the 4Fe-4S cluster domain of human DNA primase large subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-24 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 126.476, 83.724, 47.561 |
Unit cell angles | 90.00, 97.65, 90.00 |
Refinement procedure
Resolution | 31.160 - 2.500 |
R-factor | 0.228 |
Rwork | 0.228 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lgb |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.074 | 0.451 |
Number of reflections | 16851 | |
<I/σ(I)> | 20.6 | 3.1 |
Completeness [%] | 98.6 | 98.4 |
Redundancy | 3.5 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.2 M magnesium acetate tetrahydrate, 0.05 M sodium cacodylate, 15% w/v PEG8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |