3Q29
Cyrstal structure of human alpha-synuclein (1-19) fused to maltose binding protein (MBP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97938 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.390, 117.470, 146.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.532 - 2.300 |
R-factor | 0.1945 |
Rwork | 0.193 |
R-free | 0.23180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1anf |
RMSD bond length | 0.004 |
RMSD bond angle | 0.837 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.532 | 2.360 | |
High resolution limit [Å] | 2.300 | 10.290 | 2.300 |
Rmerge | 0.059 | 0.031 | 0.461 |
Number of reflections | 53017 | 604 | 3883 |
<I/σ(I)> | 16.32 | 33.2 | 3.3 |
Completeness [%] | 99.6 | 87 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 0.1M TRIS pH 8.0, 2.4 M AMMONIUM SULFATE, vapor diffusion, hanging drop, temperature 290K |