3Q1R

Crystal structure of a bacterial RNase P holoenzyme in complex with TRNA and in the presence of 5' leader

Replaces:  3OKB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-10
Detector	RAYONIX MX-225
Spacegroup name	P 31 2 1
Unit cell lengths	169.920, 169.920, 185.530
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.630 - 4.210
R-factor	0.259
Rwork	0.258
R-free	0.26700
Structure solution method	MIRAS
Starting model (for MR)	2a2e 2a64 1nz0 1ehz
RMSD bond length	0.007
RMSD bond angle	1.230
Data reduction software	XDS
Data scaling software	SCALA
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	4.390
High resolution limit [Å]	4.200	4.200
Rmerge	0.081	0.463
Number of reflections	17674
<I/σ(I)>	10.7	2.4
Completeness [%]	76.4	7.8
Redundancy	5.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	303	1.8M LiSO4, 0.5M sodium cacolydate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 303K