3Q1Q

Structure of a Bacterial Ribonuclease P Holoenzyme in Complex with tRNA

Replaces:  3OK7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-20
Detector	RAYONIX MX-225
Spacegroup name	P 31 2 1
Unit cell lengths	169.290, 169.290, 184.990
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.970 - 3.800
R-factor	0.251
Rwork	0.250
R-free	0.27200
Structure solution method	MIRAS
Starting model (for MR)	2a2e 2a64 1nz0 1ehz
RMSD bond length	0.007
RMSD bond angle	1.240
Data reduction software	XDS
Data scaling software	XDS
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.970
High resolution limit [Å]	3.800	3.800
Rmerge	0.079	0.359
Number of reflections	27116
<I/σ(I)>	12.2	3.2
Completeness [%]	88.5	22.3
Redundancy	5	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6		1.8M Lithium sulfate, 0.05M sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP