3Q1I
Polo-like kinase I Polo-box domain in complex with FMPPPMSpSM phosphopeptide from TCERG1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Collection date | 2010-11-17 |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.500, 51.500, 57.600 |
| Unit cell angles | 90.00, 101.00, 90.00 |
Refinement procedure
| Resolution | 56.540 - 1.400 |
| R-factor | 0.19844 |
| Rwork | 0.197 |
| R-free | 0.22156 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1UMW chain A |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.968 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 56.540 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 38959 |
| Completeness [%] | 97.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 30% PEG400, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
