3Q19
Human Glutathione Transferase O2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-01 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95663 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.425, 85.017, 60.397 |
| Unit cell angles | 90.00, 94.55, 90.00 |
Refinement procedure
| Resolution | 42.510 - 1.900 |
| R-factor | 0.165 |
| Rwork | 0.164 |
| R-free | 0.19418 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eem |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.133 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.510 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.054 | 0.155 |
| Number of reflections | 34258 | |
| <I/σ(I)> | 10.5 | 3.9 |
| Completeness [%] | 92.9 | 85.2 |
| Redundancy | 5.9 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 0.1M sodium chloride, 0.1M Bicine pH 9.0, 20% (v/v) PEGMME 2000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
