3Q0R
Crystal structure of the PUMILIO-homology domain from Human PUMILIO2 in complex with p38alpha NREb
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-17 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.482, 43.121, 60.612 |
| Unit cell angles | 71.93, 85.36, 77.58 |
Refinement procedure
| Resolution | 27.682 - 2.000 |
| R-factor | 0.2099 |
| Rwork | 0.207 |
| R-free | 0.25710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.122 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.4_6)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.700 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 21575 | |
| Completeness [%] | 93.1 | 79.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 293 | 17-20% (w/v) PEG 3000, 100 mM Na3Citrate pH 5.0-6.0 , VAPOR DIFFUSION, temperature 293K |
