3Q0H
Structure of T-cell immunoreceptor with immunoglobulin and ITIM domains (TIGIT)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.951, 74.671, 43.346 |
Unit cell angles | 90.00, 92.39, 90.00 |
Refinement procedure
Resolution | 43.310 - 1.700 |
R-factor | 0.2087 |
Rwork | 0.207 |
R-free | 0.23700 |
Structure solution method | MIR |
Starting model (for MR) | 1ikf |
RMSD bond length | 0.015 |
RMSD bond angle | 1.678 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
Rmerge | 0.038 | 0.027 | 0.535 |
Number of reflections | 27916 | ||
<I/σ(I)> | 16.4 | ||
Completeness [%] | 99.7 | 94.5 | 100 |
Redundancy | 7.3 | 7.2 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 1.3M Ammonium Sulfate, 0.1M MES pH 6.0, Vapor diffusion, Sitting drop, temperature 298K |