3PZS
Crystal Structure of a pyridoxamine kinase from Yersinia pestis CO92
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-02 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.914, 88.101, 102.917 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.920 - 1.890 |
R-factor | 0.172 |
Rwork | 0.170 |
R-free | 0.20100 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.000 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (AutoSolve) |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.930 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.067 | 0.611 |
Number of reflections | 49995 | |
<I/σ(I)> | 2.41 | 3.69 |
Completeness [%] | 99.7 | 98.7 |
Redundancy | 5.1 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 1.6M Ammonium Sulfate, 0.1M NaCl, Hepes pH 7.5, 2% EG, 2% BME , VAPOR DIFFUSION, SITTING DROP, temperature 298K |