3PZB
Crystals Structure of Aspartate beta-Semialdehyde Dehydrogenase complex with NADP and D-2,3-Diaminopropionate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-03 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.042, 100.090, 64.483 |
| Unit cell angles | 90.00, 100.81, 90.00 |
Refinement procedure
| Resolution | 23.000 - 2.000 |
| R-factor | 0.17889 |
| Rwork | 0.177 |
| R-free | 0.20818 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.996 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 46990 | |
| <I/σ(I)> | 10.5 | 4.2 |
| Completeness [%] | 93.2 | 70.3 |
| Redundancy | 2.8 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 23% PEG 8K, 0.1 M tris-HCL, 0.1 M Na-acetate, 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
