3PZ1
Crystal structure of RabGGTase(DELTA LRR; DELTA IG) in Complex with BMS3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-09-02 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9764 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.554, 90.583, 114.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.400 - 1.950 |
R-factor | 0.18469 |
Rwork | 0.182 |
R-free | 0.23191 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dss |
RMSD bond length | 0.010 |
RMSD bond angle | 1.171 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.050 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 50703 | |
<I/σ(I)> | 19.7 | 5.5 |
Completeness [%] | 99.0 | 98.6 |
Redundancy | 4.1 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 284 | 14% (w/v) PEG3350, 0.2 M Ca(OAc)2, 0.1 M HEPES, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 284K |