3PXM
Reduced sweetness of a monellin (MNEI) mutant results from increased protein flexibility and disruption of a distant poly-(L-proline) II helix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-04-20 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.889, 39.652, 45.153 |
| Unit cell angles | 84.90, 80.25, 83.90 |
Refinement procedure
| Resolution | 24.630 - 1.800 |
| R-factor | 0.1792 |
| Rwork | 0.177 |
| R-free | 0.21892 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iv9 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.456 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 22.430 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 17472 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.2 M Li2SO4, 0.1M HEPES and 25% w/v PEG4000, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
