3PTR

PHF2 Jumonji domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	65.737, 66.512, 104.567
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.850 - 1.954
R-factor	0.1825
Rwork	0.180
R-free	0.22930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kv4
RMSD bond length	0.006
RMSD bond angle	0.985
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.850	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.094	0.539
Number of reflections	33743
<I/σ(I)>	18.8	3
Completeness [%]	99.3	97.3
Redundancy	6.1	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6		18-30% polyethylene glycol 3350 and 100 mM NaCitrate, pH 5.2-6.0, VAPOR DIFFUSION