Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.2398 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.437, 69.122, 69.024 |
| Unit cell angles | 90.00, 105.44, 90.00 |
Refinement procedure
| Resolution | 19.171 - 2.400 |
| R-factor | 0.1973 |
| Rwork | 0.195 |
| R-free | 0.24290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z2w |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.146 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.610 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 14590 |
| Completeness [%] | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.6 | 289 | 0.1M Tris, 0.2M NaCl, 20% PEG 3350, pH 8.6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
