3PQR
Crystal structure of Metarhodopsin II in complex with a C-terminal peptide derived from the Galpha subunit of transducin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-05 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | H 3 2 |
Unit cell lengths | 241.550, 241.550, 109.870 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.860 - 2.850 |
Rwork | 0.217 |
R-free | 0.24958 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dqb |
RMSD bond length | 0.009 |
RMSD bond angle | 1.256 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.865 | 3.000 |
High resolution limit [Å] | 2.849 | 2.850 |
Rmerge | 0.059 | 0.409 |
Number of reflections | 28448 | |
<I/σ(I)> | 15.9 | 2.9 |
Completeness [%] | 99.2 | 98.6 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |