3PNL
Crystal Structure of E.coli Dha kinase DhaK-DhaL complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-15 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 74.572, 74.572, 268.779 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.920 - 2.200 |
R-factor | 0.1911 |
Rwork | 0.189 |
R-free | 0.22480 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.619 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.920 | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
Rmerge | 0.091 | 0.042 | 0.597 |
Number of reflections | 38172 | ||
<I/σ(I)> | 9.9 | ||
Completeness [%] | 96.2 | 98.3 | 75.6 |
Redundancy | 12.8 | 12.6 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 3.5 M Sodium Formate, 0.1M Hepes pH 7.5, vapor diffusion, sitting drop, temperature 295K |