3PN6
Crystal structure of Arabidopsis thaliana petide deformylase 1B (AtPDF1B) G41M mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 66.280, 66.280, 194.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.560 - 2.100 |
| R-factor | 0.17307 |
| Rwork | 0.171 |
| R-free | 0.21223 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m6o |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.527 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 26225 | |
| <I/σ(I)> | 21.56 | 6.8 |
| Completeness [%] | 99.7 | 98.2 |
| Redundancy | 9.5 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15% PEG-3350, Zinc acetate 100mM, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
