3PMN

ternary crystal structure of polymerase lambda variant with a GT mispair at the primer terminus with Mn2+ in the active site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-20
Detector	RIGAKU SATURN 92
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	56.390, 62.417, 139.881
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.270 - 2.200
R-factor	0.213
Rwork	0.213
R-free	0.24300
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.005
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	25329
<I/σ(I)>	17.6	2.4
Completeness [%]	99.2	98.2
Redundancy	3.9	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	2M formate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K