3PM8
CAD domain of PFF0520w, Calcium dependent protein kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-10-25 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.019, 51.378, 51.639 |
| Unit cell angles | 68.53, 65.75, 64.49 |
Refinement procedure
| Resolution | 26.610 - 2.000 |
| R-factor | 0.2004 |
| Rwork | 0.199 |
| R-free | 0.22520 |
| Structure solution method | SAD/MR |
| Starting model (for MR) | 3l19 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.041 | 0.024 | 0.484 |
| Number of reflections | 24816 | ||
| <I/σ(I)> | 18.8 | 3.46 | |
| Completeness [%] | 95.1 | 98.7 | 88.5 |
| Redundancy | 4 | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 25% PEG 3350, 0.2M MgCl2, 0.1M Hepes 7.5, 5mM CaCl2, 2.5mM MgCl2, glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
