3PLQ
Crystal structure of PKA type I regulatory subunit bound with Rp-8-Br-cAMPS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 66.990, 66.990, 58.860 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
Rwork | 0.249 |
R-free | 0.32750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3FHI(Chain B) |
RMSD bond length | 0.006 |
RMSD bond angle | 1.210 |
Data scaling software | SCALEPACK |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 7036 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 6.5 | 298 | pH 6.5, hanging drop, temperature 298K |