3PLF
Reverse Binding Mode of MetRD peptide complexed with c-Cbl TKB domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-10 |
| Detector | Bruker AXIOM 200 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.743, 104.483, 60.548 |
| Unit cell angles | 90.00, 90.19, 90.00 |
Refinement procedure
| Resolution | 47.080 - 1.920 |
| R-factor | 0.1602 |
| Rwork | 0.159 |
| R-free | 0.18020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bux |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.627 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.990 |
| High resolution limit [Å] | 1.920 | 4.140 | 1.920 |
| Rmerge | 0.065 | 0.057 | 0.203 |
| Number of reflections | 48545 | ||
| <I/σ(I)> | 11.3 | ||
| Completeness [%] | 97.6 | 99.9 | 92.5 |
| Redundancy | 4 | 5.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 6.5 | 298 | 100mM Bis-tris propane, 50mM ammonium sulfate, pH 6.5, hanging drop, temperature 298K |
