3PIP
Crystal structure of the synergistic antibiotic pair lankamycin and lankacidin in complex with the large ribosomal subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | PSI PILATUS 6M |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 169.723, 408.562, 693.334 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.998 - 3.450 |
| R-factor | 0.257 |
| Rwork | 0.257 |
| R-free | 0.30100 |
| Structure solution method | ISOMORPHOUS REPLACEMENT |
| Starting model (for MR) | 2zjr |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.271 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.998 | 3.570 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Rmerge | 0.216 | |
| Number of reflections | 260194 | |
| <I/σ(I)> | 5.6 | 1.4 |
| Completeness [%] | 84.3 | 85.6 |
| Redundancy | 4.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP. 15% 2:1 ethanol:methylpentanediol was diffused to a hanging drop of 180 AU/ml solution of D50S. |
