3PI8
Site-specific Glycosylation of Hemoglobin Utilizing Oxime Ligation Chemistry as a Viable Alternative to PEGylation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-07-27 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.977, 72.995, 128.483 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.060 - 2.200 |
| R-factor | 0.22907 |
| Rwork | 0.226 |
| R-free | 0.28057 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g0a |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.992 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 64.240 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 29989 |
| Completeness [%] | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch under Oil | 9.5 | 293 | PEG 2250 MME, pH 9.5, Microbatch under Oil, temperature 293K |
