3PG7

Crystal structure of the H. sapiens NF1 SEC-PH domain (del1750 mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Wavelength(s)	1.00
Spacegroup name	P 41 21 2
Unit cell lengths	113.440, 113.440, 124.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.000 - 2.189
R-factor	0.2104
Rwork	0.208
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.897
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	36.857
High resolution limit [Å]	2.100
Number of reflections	74716
Completeness [%]	93.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1