3PE3
Structure of human O-GlcNAc transferase and its complex with a peptide substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 273.400, 273.400, 142.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.583 - 2.780 |
| R-factor | 0.1852 |
| Rwork | 0.185 |
| R-free | 0.21770 |
| Structure solution method | MIR |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.707 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.930 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Number of reflections | 154231 | |
| <I/σ(I)> | 8.4 | 3 |
| Completeness [%] | 98.2 | 95.1 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 1.45 M Potassium Phosphate Dibasic, 8 mM EDTA, 1% xylitol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
