3PDR
Crystal structure of manganese bound M-box RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.780, 102.487, 126.375 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.488 - 1.853 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.22780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.428 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.890 |
| High resolution limit [Å] | 1.853 | 5.050 | 1.853 |
| Rmerge | 0.087 | 0.040 | 0.916 |
| Number of reflections | 73903 | ||
| <I/σ(I)> | 9.5 | ||
| Completeness [%] | 96.8 | 91.6 | 97.6 |
| Redundancy | 5.7 | 5.8 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.2 | 293 | 10%MPD, 40mM sodium cacodylate, 12mM Spermine .4HCl,20mM KCl, 50mM Ammonium sulfate, 5mM MnCl2, pH 7.2, VAPOR DIFFUSION, temperature 293K |
