3PCU

Crystal structure of human retinoic X receptor alpha ligand-binding domain complexed with LX0278 and SRC1 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	277
Detector technology	CCD
Collection date	2010-04-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9753
Spacegroup name	P 43 21 2
Unit cell lengths	65.370, 65.370, 110.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.360 - 2.000
R-factor	0.20099
Rwork	0.201
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2p1t
RMSD bond length	0.006
RMSD bond angle	1.095
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	31.360
High resolution limit [Å]	2.000
Number of reflections	16134

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	8% v/v Tacsimate pH8.0, 20% w/v PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K