3P9O
Aerobic ternary complex of urate oxidase with azide and chloride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 291 |
| Detector technology | CCD |
| Collection date | 2009-05-13 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 79.493, 94.993, 104.219 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.700 - 1.450 |
| Rwork | 0.201 |
| R-free | 0.25500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3l8w |
| RMSD bond length | 0.032 |
| RMSD bond angle | 0.078 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.700 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.055 | 0.233 |
| Number of reflections | 69903 | |
| <I/σ(I)> | 22.5 | 3.8 |
| Completeness [%] | 99.4 | 97.1 |
| Redundancy | 5.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10 | 291 | SOLUTION A (0.1 ML): 50MM TRIS BUFFER, 0.3 M SODIUM AZIDE, SATURATED WITH URIC ACID BUFFER, VAPOR DIFFUSION, SITTING DROP, temperature 291K, pH 10.0 |
