3P9O
Aerobic ternary complex of urate oxidase with azide and chloride
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 291 |
Detector technology | CCD |
Collection date | 2009-05-13 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 79.493, 94.993, 104.219 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.700 - 1.450 |
Rwork | 0.201 |
R-free | 0.25500 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3l8w |
RMSD bond length | 0.032 |
RMSD bond angle | 0.078 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.700 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.055 | 0.233 |
Number of reflections | 69903 | |
<I/σ(I)> | 22.5 | 3.8 |
Completeness [%] | 99.4 | 97.1 |
Redundancy | 5.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 10 | 291 | SOLUTION A (0.1 ML): 50MM TRIS BUFFER, 0.3 M SODIUM AZIDE, SATURATED WITH URIC ACID BUFFER, VAPOR DIFFUSION, SITTING DROP, temperature 291K, pH 10.0 |