3P87
Structure of human PCNA bound to RNASEH2B PIP box peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.982 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.108, 81.788, 116.558 |
Unit cell angles | 90.00, 91.47, 90.00 |
Refinement procedure
Resolution | 47.450 - 2.990 |
R-factor | 0.2241 |
Rwork | 0.223 |
R-free | 0.24850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1axc |
RMSD bond length | 0.009 |
RMSD bond angle | 1.090 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.9.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.500 | 3.090 |
High resolution limit [Å] | 2.990 | 2.990 |
Rmerge | 0.126 | 0.852 |
Number of reflections | 31807 | |
<I/σ(I)> | 8 | 1.8 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.7 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 293.15 | 3M NaCl, 0.1M citrate, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |