3P4X
Helicase domain of reverse gyrase from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.259, 111.231, 129.726 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.032 - 2.410 |
| R-factor | 0.1899 |
| Rwork | 0.186 |
| R-free | 0.25630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oiy |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.144 |
| Data reduction software | XDS ((VERSION May 10) |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 129.730 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.064 | 0.635 |
| Number of reflections | 34066 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 99.1 | 99.9 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 20% PEG3350, 0.2M Mg-formiate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
