3P4W
Structure of desflurane bound to a pentameric ligand-gated ion channel, GLIC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 181.120, 132.370, 159.690 |
Unit cell angles | 90.00, 102.13, 90.00 |
Refinement procedure
Resolution | 15.000 - 3.200 |
R-factor | 0.1877 |
Rwork | 0.187 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eam |
RMSD bond length | 0.010 |
RMSD bond angle | 1.170 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | CCP4 |
Refinement software | BUSTER (2.9.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.107 | 0.547 |
Number of reflections | 59199 | |
<I/σ(I)> | 9.1 | 2 |
Completeness [%] | 97.5 | 97.7 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |