3P42
Structure of GfcC (YmcB), protein encoded by the E. coli group 4 capsule operon
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-06 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.831, 99.980, 69.020 |
Unit cell angles | 90.00, 91.74, 90.00 |
Refinement procedure
Resolution | 34.494 - 1.910 |
R-factor | 0.1752 |
Rwork | 0.173 |
R-free | 0.21680 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.110 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER ((in PHENIX)) |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.000 |
High resolution limit [Å] | 1.770 |
Number of reflections | 81677 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277.15 | 1.5 M Ammonium Sulfate, 0.1 M NaCl, 0.1 M Bis-Tris pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |