3P42

Structure of GfcC (YmcB), protein encoded by the E. coli group 4 capsule operon

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-06
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	68.831, 99.980, 69.020
Unit cell angles	90.00, 91.74, 90.00

Refinement procedure

Resolution	34.494 - 1.910
R-factor	0.1752
Rwork	0.173
R-free	0.21680
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.110
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER ((in PHENIX))
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	49.000
High resolution limit [Å]	1.770
Number of reflections	81677
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277.15	1.5 M Ammonium Sulfate, 0.1 M NaCl, 0.1 M Bis-Tris pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K