3P2D
Crystal structure of arrestin-3 reveals the basis of the difference in receptor binding between two non-visual subtypes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97914 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.177, 73.323, 201.973 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.364 - 3.000 |
| R-factor | 0.2225 |
| Rwork | 0.219 |
| R-free | 0.28640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g4r |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.531 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 20910 | |
| Completeness [%] | 92.7 | 92.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 50mM HEPES, 675mM Na/K tartrate, pH 7.5. , VAPOR DIFFUSION, HANGING DROP, temperature 291K |
