3P27

Crystal structure of S. cerevisiae Hbs1 protein (GDP-bound form), a translational GTPase involved in RNA quality control pathways and interacting with Dom34/Pelota

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.5418
Spacegroup name	P 42 21 2
Unit cell lengths	109.964, 109.964, 188.199
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 2.950
R-factor	0.2173
Rwork	0.214
R-free	0.27750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.358
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.980	19.980	3.020
High resolution limit [Å]	2.950	3.400	2.950
Number of reflections	45371
Completeness [%]	98.2	98	97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	50mM MOPS, 12% PEG 4000, 20% Glycerol, 500mM KCl, pH 7.0, VAPOR DIFFUSION, temperature 293K