3P0L

Human steroidogenic acute regulatory protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2007-08-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97948
Spacegroup name	P 63
Unit cell lengths	144.130, 144.130, 101.030
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.908 - 3.400
R-factor	0.2598
Rwork	0.257
R-free	0.28710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1em2
RMSD bond length	0.002
RMSD bond angle	0.512
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	3.500
High resolution limit [Å]	3.400	3.400
Rmerge	0.173	0.797
Number of reflections	16316
<I/σ(I)>	19.78	4.75
Completeness [%]	98.6	100
Redundancy	22.6	22.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	277	200 mM Ca-acetate, 40 % PEG-300, 100 mM Na-Cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 277K