3OYG
Crystal structure of the Prototype Foamy Virus (PFV) intasome in complex with magnesium and the INSTI Compound1 (CompoundG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2010-06-08 |
Detector | ADSC |
Wavelength(s) | 0.98011 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 160.060, 160.060, 123.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.150 - 2.560 |
R-factor | 0.2063 |
Rwork | 0.205 |
R-free | 0.22660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3oyb |
RMSD bond length | 0.011 |
RMSD bond angle | 1.486 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.147 | 39.147 | 2.700 |
High resolution limit [Å] | 2.560 | 8.100 | 2.560 |
Rmerge | 0.030 | 0.696 | |
Total number of observations | 7391 | 24927 | |
Number of reflections | 50408 | ||
<I/σ(I)> | 9.7 | 35 | 1.6 |
Completeness [%] | 97.0 | 95.4 | 92.6 |
Redundancy | 3.9 | 4.3 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.35 M ammonium sulfate, 25% (v/v) glycerol, 4.8% (v/v) 1,6-hexanediol, 50 mM Mes-NaOH, 1mM EDTA, pH 6.5, vapor diffusion, hanging drop, temperature 291K |