3OXU
Complement components factor H CCP19-20 and C3d in complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 74.675, 82.990, 85.605 |
| Unit cell angles | 112.75, 110.14, 99.96 |
Refinement procedure
| Resolution | 40.482 - 2.100 |
| R-factor | 0.1724 |
| Rwork | 0.171 |
| R-free | 0.20590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2G7I 1c3d |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.018 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.600 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.053 | 0.465 |
| Number of reflections | 93199 | |
| <I/σ(I)> | 14.9 | 5.1 |
| Completeness [%] | 96.4 | 96.4 |
| Redundancy | 4.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 290 | 4% w/v PEG 8000, 0.1 M Tris HCl, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
