3OUG
Crystal structure of cleaved L-aspartate-alpha-decarboxylase from Francisella tularensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.934, 80.525, 85.025 |
| Unit cell angles | 90.00, 104.33, 90.00 |
Refinement procedure
| Resolution | 28.660 - 1.550 |
| R-factor | 0.17196 |
| Rwork | 0.172 |
| R-free | 0.19704 |
| Structure solution method | SAD with MR |
| Starting model (for MR) | 2c45 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.510 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.071 | 0.663 |
| Number of reflections | 150740 | |
| <I/σ(I)> | 27.6 | |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.2 M Lithium sulfate, 0.1 M Bis-Tris, 25% PeG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
