3OTD

Crystal structure of human tRNAHis guanylyltransferase (Thg1)- NaI derivative

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	104
Detector technology	IMAGE PLATE
Collection date	2009-04-10
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	140.574, 140.574, 80.962
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.280
Rwork	0.236
R-free	0.25840
Structure solution method	SIRAS
RMSD bond length	0.007
RMSD bond angle	1.178
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	15.000	15.000	2.360
High resolution limit [Å]	2.280	4.860	2.280
Rmerge	0.087	0.041	0.804
Number of reflections	37206
<I/σ(I)>	9.6
Completeness [%]	99.9	99.9	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	7.5	291	PEG 3350, MgSO4, pH 7.5, hanging drop, temperature 291K