3OSK

Crystal structure of human CTLA-4 apo homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	43.864, 51.457, 102.849
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.012 - 1.800
R-factor	0.2032
Rwork	0.200
R-free	0.27510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i8l chain C
RMSD bond length	0.010
RMSD bond angle	1.223
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.800
Rmerge	0.111
Number of reflections	22221
<I/σ(I)>	10.33
Completeness [%]	99.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	295	25% Polyethylene glycol 3350 (w/v), 100mM bis-Tris pH 5.5, 200mM ammonium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 295K