Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9766 |
| Spacegroup name | P 64 |
| Unit cell lengths | 200.327, 200.327, 52.060 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.200 - 1.860 |
| Rwork | 0.209 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xcl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.970 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.200 | 1.890 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.700 | 0.407 |
| Number of reflections | 97258 | |
| <I/σ(I)> | 21.2 | 2.3 |
| Completeness [%] | 96.5 | 74 |
| Redundancy | 7.7 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 297 | 21% PEG3350, 0.1M CACL2, 0.1M TRIS, 1MM GUANIDINE HCL, pH 6.5, VAPOR DIFFUSION, temperature 297K |
