3ORF
Crystal Structure of Dihydropteridine Reductase from Dictyostelium discoideum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-07 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 2.16 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.808, 129.898, 78.763 |
Unit cell angles | 90.00, 99.99, 90.00 |
Refinement procedure
Resolution | 36.960 - 2.160 |
R-factor | 0.18771 |
Rwork | 0.185 |
R-free | 0.24014 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dhr |
RMSD bond length | 0.023 |
RMSD bond angle | 1.926 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.000 | 2.240 |
High resolution limit [Å] | 2.160 | 2.160 |
Rmerge | 0.069 | 0.423 |
Number of reflections | 46917 | |
Completeness [%] | 93.4 | 96.4 |
Redundancy | 6.9 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 17% PEG 3350, 0.1M bis-tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |