3OQ7
Crystal Structures of Multidrug-Resistant Clinical Isolate 769 HIV-1 Protease Variants
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-11 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 45.534, 45.534, 101.926 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.230 - 1.710 |
| R-factor | 0.19646 |
| Rwork | 0.195 |
| R-free | 0.22337 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tw7 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.378 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.230 | 1.800 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Number of reflections | 11367 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 100 | |
| Redundancy | 5.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.3-1.0M sodium chloride in the pH range 5.5-7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
